Objective To establish a real-time fluorescent quantitative ＲT-PCＲ assay to detect tree threw ( tupaiabelangeri) IL-2． Methods Total ＲNA was isolated from Con A-stimulated tree shrews' spleen lymphocytes． The conservative encoding sequence of interleukin-2 ( IL-2) was amplified by ＲT-PCＲ，and successfully cloned into pMD-19T vector． The established IL-2 gene vector was used as standard substance and the standard curves were established for the sensitivity evaluation． Ｒesults A real-time fluorescence quantitative method of fast，sensitive and reliable detection system was set up． The sensitivity of this method for creating IL-2 gene of tree shrew was 102 ～ 109copies． Conclusions A realtime fluorescence quantitative method of tree shrew IL-2 was successfully established． This sensitive method can serve as a molecular foundation for the study of IL-2 and its role in many clinical diseases．