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Home > Archive>Volume 24, Issue 3, 2014 >34-38. DOI:10.3969.j.issn.1671.7856.2014.003.008
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Establishment of sperm specific Sleeping Beauty transposase-expressing transgenic mouse
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    Abstract:

    Objective To establish the sperm specific Sleeping Beauty (SB) transposase-expression transgenic mouse for the study of the genetic modification mediated by transposon system in mouse. Methods Prm1 promoter was cloned from mouse genomic DNA to drive the expression of SB transposase. The transgenic mice were generated by microinjection. The gene type of transgenic line was identified by PCR. The expressing level in testis was determined by western blot and immunohistochemistry (IHC) staining. Results Five lines of transposase transgenic mice were obtained by microinjection and three can be germline. One mouse line with higher expression level of transposase in the testis was obtained. Conclusion One transgenic mouse model with Sleeping Beauty transposase-expression was successfully established. This model will greatly contribute to the research of genetic modification mediated by transposon in mouse

    Reference
    [1] Lander ES, Linton LM, Birren B, et al. Initial sequencing and analysis of the human genome[J]. Nature, 2001. 409(6822): 860-921.
    [2] Horie K, Kuroiwa A, Ikawa M, et al. Efficient chromosomal transposition of a Tc1/mariner-like transposon Sleeping Beauty in mice[J]. Proc Natl Acad Sci U S A, 2001. 98(16): 9191-9196.
    [3] Wilber A, Linehan JL, Tian X, et al. Efficient and stable transgene expression in human embryonic stem cells using transposon-mediated gene transfer[J]. Stem Cells, 2007. 25(11): 2919-2927.
    [4] Ding S, Wu X, Li G, et al. Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice[J]. Cell, 2005. 122(3): 473-483.
    [5] Ivics Z, Hackett PB, Plasterk RH, et al. Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells[J]. Cell, 1997. 91(4): 501-510.
    [6] Izsvak Z, Ivics Z. Sleeping beauty transposition: biology and applications for molecular therapy[J]. Mol Ther, 2004. 9(2): 147-156.
    [7] Hackett PB, Ekker SC, Largaespada DA, et al. Sleeping beauty transposon-mediated gene therapy for prolonged expression[J]. Adv Genet, 2005. 54: 189-232.
    [8] 马元武, 张连峰. 转座子 Sleeping Beauty 和 PiggyBac[J]. 中国生物化学与分子生物学报, 2010(9): 783-787.
    [9] Geurts AM, Wilber A, Carlson CM, et al. Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposon[J]. BMC Biotechnol, 2006. 6: 30.
    [10] 马元武, 廉虹, 王冬梅, 等. 一种包含转座子 Sleeping Beauty 和 PiggyBac 的新型多功能载体的构建[J]. 中国生物化学与分子生物学报, 2012. 2: 182-189.
    [11] Birnboim HC. A rapid alkaline extraction method for the isolation of plasmid DNA[J]. Methods Enzymol, 1983. 100: 243-255.
    [12] Fischer AH, Jacobson KA, Rose J, et al. Hematoxylin and eosin staining of tissue and cell sections[J]. CSH Protoc, 2008. 2008: pdb prot4986.
    [13] Gaj T, Gersbach CA, Barbas CF. ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering[J]. Trends Biotechnol, 2013. 31(7): 397-405.
    [14] Mátés L, Chuah MKL, Belay E, et al. Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates[J]. Nat Genet, 2009. 41(6): 753-761.
    [15] Grabundzija I, Irgang M, Belay E, et al. Comparative analysis of transposable element vector systems in human cells[J]. Mol Ther, 2010. 18(6): 1200-1209.
    [16] Ammar I, Gogol-Döring, Miskey C, et al. Retargeting transposon insertions by the adeno-associated virus Rep protein[J]. Nucleic Acids Res, 2012. 40(14): 6693-712.
    [17] Moldt B, Yant SR, Andersen PR, et al. Cis-acting gene regulatory activities in the terminal regions of sleeping beauty DNA transposon-based vectors[J]. Hum Gene Ther, 2007. 18(12): 1193-1204.
    [18] Walisko O, Schorn A, Rolfs F, et al. Transcriptional activities of the Sleeping Beauty transposon and shielding its genetic cargo with insulators[J]. Mol Ther, 2008. 16(2): 359-369.
    [19] Newman JC, Bailey AD, Fan HY, et al. An abundant evolutionarily conserved CSB-PiggyBac fusion protein expressed in Cockayne syndrome[J]. PLoS Genet, 2008. 4(3): e1000031.
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  • Revised:October 08,2013
  • Online: April 08,2014
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