Objective To explore the basic ingredients of the tree shrew's(Tupaia belangeri)milk and compare with the dairy ingredients of other milks. Methods We select ten seed tree shrews after delivery (1 ～ 21) d with lactation mother tree shrews, and use artificial passive breastfeeding method let the young tree shrews suck breast milk,we took the milk from the young tree shrews in the stomach, directly using aseptic operation with a syringe immediately, once every two days, for consecutive three to five times, and a total of 18 mL milk was taken from each seed tree shrew. Then the milk was detected according to the national standard method for component testing. Results The total solid content of the tree shrew's milk was 43.63%, including 26.01% of fat, 10.41% of protein, 0.45% of lactose and 0.99% of ash content. Compared with cow's milk, the tree shrew's milk contained 3.36 times of total solid contents, 1.24 times of ash, 2.74 times of protein, 6.67 times of fat, and 0.09 times of lactose.Compare with baby formula milk, the tree shrew's milk contained 1.44 times of total solid contents, 0.20 times of ash, 0.58 times of protein, 1.53 times of fat, and 0.06 times of lactose. The trace mineral composition of the tree shrew's milk showed that the calcium, phosphorus, potassium, sodium, magnesium, and iron contents were 1.83 times, 2.73 times, 1.25 times, 1.93 times, 1.28 times, and 1.48 times higher than those in the cow's milk, and were 0.66 times, 0.85 times, 0.34 times, 0.26 times, 0.85 times, 0.24 times lower than those in baby formula milk. Conclusions The main nutrients of tree shrew's milk is of high fat, high protein and low sugar, and it can provide a basis for tree shrews artificial brood and breeding work.