Objective To establish ectopic colon tumor model in GFP nude, make a comparative study of the differences between tumor morphology, biological characteristics and pathology with ectopic colon tumor model in BALB/c nude. Methods Use KM12SM colon cancer cells of the logarithmic growth phase seeded in right axillary subcutaneous on the GFP nude and BALB/c nude. Observed time of tumor formation, tumor volume, pathological conditions and other. Results The average time of tumor formation in GFP nude was 4~5 d, BALB/c nude was 6~8 d; The first 20 days after inoculation, tumor volume of BALB/c nude group was about(3463.43±341.39)mm³, GFP nude was about(4923.46±521.72)mm³. There are significant differences. Tumor cells growth activity, have relatively uniform size, some areas visible glandular formation on the microscope. Conclusion By comparison study, the GFP nude have certain advantages on the time of tumor formation, quality and other aspects in colon tumor model than the BALB/c nude. Provide more desirable animal model for investigating the mechanism of colon cancer lymph node metastasis, diagnosis and treatment.