Objective To screen genotypes of small body in Tibet minipigs by single nucleotide polymorphism analysis (SNP) of growth hormone (GH) gene. Methods The PCR products are sequenced. According to the results, SNP analysis are applied in GH gene 5' fragment of 108 Tibet minipigs. Results Five mutation points were found by comparison. The polymorphism information content of the T45C locus is highest. The results of growth traits of different genotypes showed that the abdomen of 6～8 month old Tibet minipigs of TC genotype at T45C mutation site is small; the weight and height of 3～5 month old Tibet minipigs of AA genotype at G84A mutation site is small; the body length and height of 6～8month old Tibet minipigs of GG genotype at G93A mutation site is small. Conclusion The AA TC, GG genotypes of T45C, G84A, G93A mutation sites in GH gene may be associated with small body. We also found that genetic mutations always occur in the above sites with high heterozygosity and genetic diversity, which can provide rich material for breeding research.