WANG Shu-jing
National Institute for Food and Drug Control, Institute for Laboratory Animal Resources, National Center for Monitoring of Laboratory Animal Health, Beijing 100050, ChinaFU Rui
National Institute for Food and Drug Control, Institute for Laboratory Animal Resources, National Center for Monitoring of Laboratory Animal Health, Beijing 100050, ChinaLI Xiao-bo
National Institute for Food and Drug Control, Institute for Laboratory Animal Resources, National Center for Monitoring of Laboratory Animal Health, Beijing 100050, ChinaWANG Ji
National Institute for Food and Drug Control, Institute for Laboratory Animal Resources, National Center for Monitoring of Laboratory Animal Health, Beijing 100050, ChinaWEI Li
National Institute for Food and Drug Control, Institute for Laboratory Animal Resources, National Center for Monitoring of Laboratory Animal Health, Beijing 100050, ChinaGONG Wei
National Institute for Food and Drug Control, Institute for Laboratory Animal Resources, National Center for Monitoring of Laboratory Animal Health, Beijing 100050, ChinaYUE Bing-fei
National Institute for Food and Drug Control, Institute for Laboratory Animal Resources, National Center for Monitoring of Laboratory Animal Health, Beijing 100050, ChinaHE Zheng-ming
National Institute for Food and Drug Control, Institute for Laboratory Animal Resources, National Center for Monitoring of Laboratory Animal Health, Beijing 100050, ChinaObjective To establish multiplex PCR assay for detection of chicken embryo lethal orphan virus(CELO)and egg drop syndrome virus (EDS). Methods According to GenBank gene sequence, two pairs of specific primers designed were amplified CELO long fiber protein and EDS hexon protein gene sequence. The specificity and sensitivity of multiplex PCR were tested. We also use the multiplex PCR to detect exogenous CELO and EDS in influenza virus. Results Two target bands have been successfully amplified and verified by sequencing. The specificity of the method is better, and the sensitivity is 10-4μg/mL. The results of detecting exogenous CELO and EDS in 12 influenza virus were negative. Conclusion The multiplex PCR assay for detection of CELO and EDS was established successfully, which have good specificity and high sensitivity, and have high value and application prospect for detecting exogenous CELO and EDS in influenza virus.
