Breeding and quality control of SPF nude mice involves many links and works. The quality and quantity of animals are directly affected by breeding management and quality control. A standardized breeding system of SPF BALB/c nude mice were gradually established at the center after 2007. This paper introduces specific practices and experience in facilities, stock, breeding management, and quality control of SPF BALB/c nude mice, in order to provide the reference for related breeding and study in SPF BALB/c nude mice.