Caenorhabditis elegans (C. elegans), has developed into an useful model for biomedical research, particularly in the targets of novel drugs and the related mechanism. C. elegans, combining with cost-effective cultivation, simple biological structure, make for high-throughput screenings in vivo model. In this review, we explore the feasibility of C. elegans in drug activity evaluation of complex systems. Because of the body independence of C.elegans, it provided good platform for the efficacy and mechanism of research on new drugs as well as Chinese herbal extract in vivo, showing a good prospect.