Objective To assess the protective of Curcumin and Tea Polyphenols against different time of UVB-induced acute photo damage in hairless mice skin. Methods Thirty six BALB/C hair1ess mice were randomly divided into control groups and treatment groups(all of them were divided into 0 s, 30 s, 60 s, 120 s, 240 s groups with two hairless mice).Curcumin and Tea Polyphenols were applied to the mice’s skin before 30min irradiation. The irradiation distance was 15cm. The mice in each group were given three times of UVB MED about 200～540 mJ/cm² and the irradiation time were 30 s、60 s、120 s、240 s. After irradiation, the skin on the back of the radiation center were taken into paraffin section for light microscopy observation. Results Different amount of squamae appeared in the control group of hairless rat, and lymphocytes scattered in collagen tissues. In the 120 s and 240 s UVB irradiated group, the collagen was red stained, disorderly arranged, even homogenized. In the treatment group, inflammatory cell infiltration was lessened, and collagen damage weakened. Conclusion By weakening inflammatory cell infiltration and collagen damage, topical application of Curcumin and Tea Polyphenols can protect hairless mice from acute photodamage caused by UVB irradiation.