Objective To compare coagulation data measured with domestic produced and imported coagulation testing solutions on SD rat and human by testing prothrombin time(PT), activated partial thromboplastin time(APTT), thrombin time(TT), fibrinogen(FIB). Methods Blood samples were obtained from SPF SD rat and human. Domestic produced and imported coagulation testing solutions were applied to test PT, APTT, TT, and FIB. Results Compared to rat data measured with imported coagulation testing solution, data measured with domestic produced coagulation testing solution of PT, APTT, FIB was significantly higher(P<0.05), while, data of TT was statistically lower(P<0.05), and there was no obvious difference in human blood coagulation. Conclusion The data measured with different coagulation testing solution varies on SD rat, so the laboratories are required to establish reference data according to different products.