Objective To get the full-length HGF,Hepatocyte growth factor, cDNA encoding sequence and predict its molecular characteristics. Methods Full-length HGF cDNA encoding sequence is cloned from total RNA in liver tissue of tree shrew by RT-PCR and its molecular characteristics is analyzed compared with other mammals by using biology software such as DNAMAN,Pymol and so forth. Results A 2193bp of the full-length HGF cDNA encoding sequence is cloned from total RNA in liver tissue of tree shrew.The analysis of molecular characteristics show that the molecular structure of HGF is relatively conservative in evolution, tree shrew HGF overall structure similar to Homo sapiens.However,compared with the structure and charge surface of NK1 domain of HGFα-chain of human,tree shrew has two longer β-sheet and one more α-helix,Additional,tree shrew had a strong positive charged site in this area,which may have a impact on the bind of HGF to its receptor. Conclusion This study provide a theoretical basis for preparation and receptor-binding mechanism studies of tree shrew HGF.