Objective To evaluate the safety of Light emitting diode (LED) source through irradiating the skin of Japanese big-ear white rabbits. Methods Animals were randomly divided into irradiated group and control group. Animals in the two groups were irradiated with LED light source after eliminating back hair (50mw/cm²) and the ordinary fluorescent lamp, respectively. The animals were irradiated for 5 hours every day for 3 months. The changes of animal skin symptoms, feeding, body weight, and temperature were observed and detected; the blood routine and biochemical indexes were tested; the changes of haematological immune cells and cytokines were analyzed by ELISA; histopathology and elastic fibers of skin were detected; the levels of C-myc, P53, and cyclin D1 in skin were tested by immunohistochemistry. Results The skin of rabbits in irradiated group showed no abnormal change; the weight was higher than the control group animals at the 6th week, and the temperature had difference at the 3rd and 6th week, the diet showed no abnormal. Blood routine showed white blood cells increased; biochemical detection manifested urea and creatinine levels increased, but the indexes are in normal range; the hematological CD3T cells, cytokines IL-6,TNF-α and IFN-γ in the two group animals had no significant difference. The levels of CD19B cell and IL-4 level increased in irradiated group; The viscera histopathology structure and skin elastic fibers distribution in irradiated group animals showed normal; the expression of cell cycle protein 1 (CCND1), c-myc protein and P53 protein in skin of irradiated group had no difference with control. Conclusions After irradiating 3 months, the LED light source had not caused the obvious pathology damage of tissues and change of skin tumor related factors to the rabbits.