Objective To investigate the effect of Icariin (ICA) experimental IgA nephropathy in rats and to explore related mechanisms. Methods Experimental IgA nephropathy rat model was established and then model rat were treated with or without different doses of ICA. Then, urine RBC, Urine protein and urine NAG were analyzed; IgA precipitation was detected with immunofluorescence staining; the protein level of NF-κBp65 and MCP-1 were examined by immunohistochemical staining; the mRNA level of IL-4, IL-10 and IL-13 were determined by quantitative PCR. Results The concentrations of urine RBC, Urine protein and urine NAG were reduced after ICA treatment, as companied by a decrease of IgA precipitation. Moreover, ICA treatment also decreased the protein level of NF-κBp65 and MCP-1, and the mRNA level of IL-4, IL-10 and IL-13. Conclusions ICA exerts a certain degree of efficacy on the treatment of experimental IgA nephropathy through regulating NF-κBp65 and MCP-1 expression and the immunoregulation mechanism.