Optimization of steam sterilization conditions for animal cages and beddings
CSTR:
Author:
  • Article
  • | |
  • Metrics
  • |
  • Reference [8]
  • | | | |
  • Comments
    Abstract:

    Objective To evaluate the autoclave sterilization effect on several kinds of animal cages and animal bedding, and provide the basis for safe sterilization operation. Methods Beddings and cages were sterilized with three different methods separately, and chemical indicator cards and biological indicator (Bacillus steatothermophilus) in (ATCC-7953) 3MTM pressure-steam sterilization were used to indicate the sterilization effects. Results The beddings were not completely dried when it was sterilized in whole package. The sterilization quality was unstable while the beddings were autoclaved after divided into separate cages. The best sterilization effect and dry beddings were obtained if the whole package was autoclaved at first and then divided into separate cages and sterilized again. Conclusions The results of this study demonstrate that dry beddings and best sterilization effect can be achieved by whole package autoclaved at first followed by sterilization of the bedding material divided into separate cages.

    Reference
    [1] 《病原微生物实验室安全管理条例》. 中华人民共和国国务院令第424号,2004.11.12
    [2] 李学勇,靳洪涛,刘欣. 实验动物屏障设施管理中的几个关键问题[J].中国比较医学杂志, 2008, 18(1):71-74.
    [3] 张志,王晖. 啮齿类实验动物垫料的选择和消毒[J]. 动物科学与动物医学杂志, 2004, 12(21):37.
    [4] 蔡月花,刘军须,刘福英,等. 玉米秸作为大鼠垫料对其生长发育及繁殖性能的影响[J]. 中国比较医学杂志, 2003, 13(5):282-283.
    [5] 刘福英,王春梅,刘军须,等. 5 种垫料物质的细胞毒性研究[J]. 中国比较医学杂志, 2013, 13(6):351-352.
    [6] 蒋虹,乔红伟,张汉军,等. 动物生物安全实验室废料压力蒸汽灭菌可靠性[J]. 中国消毒学杂志, 2011, 28(1):97-98.
    [7] 果海青,尉效军,左风亭. 脉动真空压力灭菌器灭菌效能的评价[J]. 中华医院感染学杂志, 2001, 11(3):209.
    [8] 恽时锋,朱虹. 在生命科学研究中影响动物实验结果的多因素分析[J]. 医学研究生学报, 2003, 16(5):372.
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation
Share
Article Metrics
  • Abstract:2626
  • PDF: 2374
  • HTML: 0
  • Cited by: 0
History
  • Revised:July 28,2016
  • Online: April 07,2017
Article QR Code