Cell biology is a highly experimental discipline, and is a discipline with theoretical knowledge closely combined with practical operation. Most of the medical graduate students should master the cell biology theory in the graduate stage, and get on a series of cell biology researches. Therefore, it is important to master the most basic cell culture techniques. As a technical instructor in the cell culture laboratory, I have been summarizing and optimizing the teaching content and teaching mode during the past few years. The aim of the teaching work is to train the students' scientific attitude of seeking truth from facts so as to develop good experimental habits and to master the basic skills of cell culture.