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Home > Archive>Volume 28, Issue 2, 2018 >26-32. DOI:10.3969. j. issn. 1671 -7856. 2018. 02. 005
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Hematopoietic stem cell injury in mice induced by busulfan
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(Institute of Laboratory Animal Science,Chinese Academy of Medical Science & Comparative Medicine Center, Peking Union Medical College,Key Laboratory of Human Disease Comparative Medicine,Ministry of Health,Beijing 100021,China)

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R-33

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    Abstract:

    Objective To study the hematopoietic stem cell injury(HSC) induced by busulfan. Methods C57BL/6 mice were treated with i. p. injection of 20 mg/ kg or 40 mg/ kg busulfan. All mice were euthanized at 15 days and 30 days after busulfan treatment for analysis of the peripheral blood cell counts, bone marrow cellularity and HPC (LKS-, lineage-sca1- c-kit+ ), HSC (LSK, lineage-sca1+ c-kit+ ) and long-term HSC (CD34- LSK, CD34- lineage-sca1 + c-kit+ ) frequency. The colony-forming unit-granulocyte and macrophage (CFU-GM) ability of HPC was measured by colony-forming cell (CFC) assay, and the HSC self-renewal capacity was analyzed by single-cell colony-forming assay. Results The busulfan administration decreased the WBC, RBC and PLT compared with control mice. The HPC function (CFU-GM) was impaired (P < 0.05), and the HSC colony forming ability was decreased at 15 days after busulfan treatment (P < 0.05), whereas the body weight of the mice didn’ t change significantly after busulfan treatment. Conclusions Our findings suggest that busulfan can induce hematopoietic stem cell injury, and provide a support for the study of hematopoietic stem cell injury mechanism.

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History
  • Received:September 08,2017
  • Online: March 14,2018
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