Objective A state quo survey of laboratory animal resource in Guangdong province is performed to provide reference data for government management decision-making and market assessment of laboratory animals. Methods We used questionnaires focusing on the laboratory animal facilities with authorization by Guangdong Province Government, which mainly included the production and use of laboratory animals, the qualification of employees and the facilities space. Results The total production and use of laboratory animals (except for the eggs) had been increasing in the last four years. 1.57 million laboratory animals were produced and 0.754 million laboratory animals were used in 2016. There were 2352 employees, roughly the same as in 2015. The facilities space for breeding was 121008 m² , and for animal experiment was 73470 m² , which were rising in the past three years. Conclusions In order to reinforce the industry development of laboratory animals in Guangdong province, some suggestions were given in our study, such as facilitating the application of superiority resource including non-human primate and aquatic laboratory animals, supporting the standardization production of several scarce mice and rats, improving relevant employees’ overall level and constructing laboratory animal facilities sharing platform.