Objective To analyze the result of proficiency testing (PT) of detection activities for Laboratory animal pathogenic bacteria in 2011 and 2013 -2017. To further improve the detection capacity of laboratory animal testing agency, and promote PT to be carried out in future. Methods During the six years (2011 and 2013 - 2017), the National Institutes for Food and Drug Control conducted a total of six (seven projects) PT activities of laboratory animal pathogen bacteria. We analyzed the overall trend and the exposed problems by summarizing the result data of the PT in 6 years. Results A total of 45 laboratories in the country including 20 provinces and cities participated in the PT. The PT projects included Mycoplasma pulmonis, Clostridium piliformis, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella spp. , Klebsiella pneumoniae and Bordetella bronchiseptica. The satisfaction rates were 75%, 87.5%, 80.0%,78.6%, 93.3, 96.2% and 88.0%, respectively. The main reasons of unsatisfactory results were for lack of incubation time, select errors of suspicious bacteria, biochemical identification errors, report writing errors and not timely feedback results. Conclusions The level of domestic laboratory animal pathogenic bacteria detection is gradually increased to achieve the desired goal through continuous proficiency testing activities.