An improved method of collecting cerebrospinal fluid in SD rats

doi:10.3969/j. issn. 1671 -7856. 2018. 04. 020

Home > Archive>Volume 28, Issue 4, 2018 >113-115. DOI:10.3969/j. issn. 1671 -7856. 2018. 04. 020

An improved method of collecting cerebrospinal fluid in SD rats
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                        10.3969/j. issn. 1671 -7856. 2018. 04. 020
                    
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                        ZHU YixinZHU Yixin
1. Department of Neurology, Affiliated Zhongda Hospital of Southeast University, Nanjing 210009, China. 2. The Institute of Neuropsychiatric Research of Southeast University, Nanjing 210009
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YE DongqingYE Dongqing
1. Department of Neurology, Affiliated Zhongda Hospital of Southeast University, Nanjing 210009, China. 2. The Institute of Neuropsychiatric Research of Southeast University, Nanjing 210009
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LI XiaoliLI Xiaoli
1. Department of Neurology, Affiliated Zhongda Hospital of Southeast University, Nanjing 210009, China. 2. The Institute of Neuropsychiatric Research of Southeast University, Nanjing 210009
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Affiliation:(1. Department of Neurology, Affiliated Zhongda Hospital of Southeast University, Nanjing 210009, China. 2. The Institute of Neuropsychiatric Research of Southeast University, Nanjing 210009)
Clc Number:R-33
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Abstract:

Objective To explore a rapid, convenient and non-polluting method of collecting cerebrospinal fluid (CSF) in Sprague Dawley (SD) rats for beginners. Methods Forty-five SD rats were divided into three groups — group 1 (collecting CSF through neck muscles), group 2 (collecting CSF through skin directly), and group 3 (collecting CSF through endorhachis). Results The successful rate of the CSF collection in the group 1 was 86.7%, group 2 was 46.7%, and group 3 is 33.3%. Conclusions The modified collection method of CSF by cisterna puncture through neck muscles in SD rats is simple, convenient, efficient and non-polluting, and is especially suitable for beginners.

Key words:rats; cerebrospinal fluid; collection method

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Received:October 13,2017
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Online: May 22,2018
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