Changes in the expression of CD169 on peripheral blood CD14 <sup>+</sup> monocytes of rhesus monkeys after SIV infection

doi:10.3969/j. issn. 1671 -7856. 2018. 05. 014

Home > Archive>Volume 28, Issue 5, 2018 >75-80. DOI:10.3969/j. issn. 1671 -7856. 2018. 05. 014

Changes in the expression of CD169 on peripheral blood CD14 + monocytes of rhesus monkeys after SIV infection
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                        10.3969/j. issn. 1671 -7856. 2018. 05. 014
                    
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                        LI XiangLI Xiang
Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health and Family Planning Commission of the People’s Republic of China (NHFPC); Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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XUE JingXUE Jing
Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health and Family Planning Commission of the People’s Republic of China (NHFPC); Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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CHEN TingCHEN Ting
Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health and Family Planning Commission of the People’s Republic of China (NHFPC); Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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CONG ZheCONG Zhe
Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health and Family Planning Commission of the People’s Republic of China (NHFPC); Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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WEI QiangWEI Qiang
Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health and Family Planning Commission of the People’s Republic of China (NHFPC); Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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Affiliation:(Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health and Family Planning Commission of the People’s Republic of China (NHFPC); Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China)
Clc Number:R-33
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Abstract:

Objective To investigate the changes of CD169 expression on the surface of peripheral blood monocytes and different subsets of monocytes in normal rhesus monkeys after SIVmac239 infection and the possible reasons. Methods Normal rhesus monkeys were infected with SIVmac239 through intravenous injection, and changes in the percentage of peripheral blood monocytes and the expression of CD169 before and after SIVmac239 infection were detected by flow cytometry. The peripheral blood CD14 ⁺ monocytes of normal rhesus monkeys sorted by flow cytometry were directly infected by SIVmac239 and stimulated by different cytokines, and changes in the expression of CD169 on the cell surface and the cytokine IFN-α were detected by flow cytometry. Results After SIVmac239 infection, the percentage of CD14 ⁺ monocytes of the normal rhesus monkeys was decreased and the expression of CD169 on their surface was increased. Meanwhile, the expression of CD169 on the surface of different subsets of peripheral blood monocytes was significantly increased, and the expression of CD169 on the CD14 ⁺ CD16 ^{+ +} monocytes was increased more obviously. CD169 was not expressed on the surface of peripheral blood CD14⁺ monocytes of the normal rhesus monkeys after stimulated by the cytokines M-CSF, IL-4 and IL-13. However, CD169 was highly expressed after the monocytes were stimulated by the cytokine IFN-α. The expression of CD169 on the surface of CD14 ⁺ monocytes and the intracellular cytokine IFN-α was not significantly changed after the monocytes were directly infected with SIVmac239. Conclusions SIVmac239 infection can lead to the increase of CD169 expression on the surface of peripheral blood monocytes in rhesus monkeys. Its expression is not associated with the direct infection of virus, but is related to the cytokine IFN-α secreted by other cells of the monkeys in vivo.

Key words:simian immunodeficiency virus, SIV; monocytes; IFN-α; CD169

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Received:November 15,2017
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Online: June 26,2018
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