+Advanced Search
Home > Archive>Volume 28, Issue 6, 2018 >101-105. DOI:10.3969/j. issn. 1671 -7856. 2018. 06. 018
PDF HTML XML Export Cite reminder
Preparation and identification of rabbit anti⁃marmoset IgG⁃HRP conjugate antibody
CSTR:
[cstr]
Author:
Affiliation:

(Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical Collage (PUMC), Beijing 100021, China)

Clc Number:

R-33

  • Article
    • | |
  • Metrics
    • | | |
  • Cited by
    • | |
  • Comments
    Abstract:

    Objective To purify marmoset serum IgG, prepare and identify the antiserum and the rabbit anti?marmoset antibody IgG?HRP (horseradish peroxidase). Methods Using SDS?PAGE analysis to identify the serum IgG from HiTrap TM Protein G. The antiserum titer was determined by double immunodiffusion assay. The rabbit anti?marmoset IgG was labeled with HRP by improved sodium periodate method. ELISA and western blotting were used to evaluate the concentration and specificity of rabbit anti?marmoset IgG?HRP. Results The purity of purified marmoset serum IgG determined by SDS?PAGE was higher than 95%, and the anti?serum titer of the anti?marmoset IgG polyclonal antibody was 1∶ 64. The concentration of rabbit anti?marmoset IgG?HRP identified by direct ELISA was 1∶ 256 000, and that by western?blotting was 1∶ 15 000, with a strong specificity. ConclusionsThe IgG?HRP marker antibody is prepared and the specificity and concentration are identified by ELISA and western blotting. It reserves the resources for the detection system of marmoset pathogens and the molecular immunological testing system.

    Reference
    Related
    Cited by
Get Citation

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:October 24,2017
  • Online: July 20,2018
Article QR Code
You are the first3023304 Visitors
® 2025 All Rights Reserved
Address：5 Panjiayuan Nanli, Chaoyang District, Beijing 100021, China Postcode： Phone：86-10-67779337 E-mail:bjb@cnilas.org
Supported by:Beijing E-Tiller Technology Development Co., Ltd.