Objective To explore expression of GLUT?1 and HIF?1α in human colorectal carcinoma tissues and discuss its clinical significance. Methods Fifty specimens of colorectal carcinoma tissues ( cancer group) and 50specimens of normal adjacent tissues (normal group) were selected, and immunohistochemistry was used to detect the expression levels of GLUT?1 and HIF?1α in the two groups. Correlations between their expression levels and clinicopathological characteristics were analyzed, and the mechanism of carcinogenesis was investigated. Results Relative expression levels of GLUT?1 in cancer and normal groups were (2.285 ±0.459) and (1.086 ±0.148), respectively, and the difference was statistically significant (t =8.919, P <0.001). The relative expression levels of HIF?1α in cancer and normal groups were (2.203 ±0.401) and (1.055 ± 0.127), respectively, and the difference was statistically significant (t = 7.144, P < 0.001). GLUT?1 expression levels were positively correlated with expression levels of HIF?1α in colorectal carcinoma tissues (r = 0.958, P < 0.001). Expression of GLUT?1 and HIF?1α was correlated with the TNM stage, depth of tumor invasion, differentiation degree, lymph node metastasis, liver metastasis, and vessel invasion ( P <0.05). Conclusions The expression levels of GLUT?1 and HIF?1α are higher in colorectal carcinoma tissues, which serve as prognostic indictors for colorectal carcinoma.