In vitro culture of dopamine neurons from newborn tree shrew

doi:10.3969/j. issn. 1671 -7856. 2018. 07. 014

Home > Archive>Volume 28, Issue 7, 2018 >82-86. DOI:10.3969/j. issn. 1671 -7856. 2018. 07. 014

In vitro culture of dopamine neurons from newborn tree shrew
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                        10.3969/j. issn. 1671 -7856. 2018. 07. 014
                    
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                        LI Juan1LI Juan
Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College; Yunnan Key Laboratory of Major Infectious Diseases Vaccine Development, Kunming 650118, China.
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ZENG Xiaofeng2ZENG Xiaofeng
School of Forensic Medicine, Kunming Medical University, Kunming 650500
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YANG Genmeng2YANG Genmeng
School of Forensic Medicine, Kunming Medical University, Kunming 650500
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TONG Pinfen1TONG Pinfen
Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College; Yunnan Key Laboratory of Major Infectious Diseases Vaccine Development, Kunming 650118, China.
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DAI Jiejie1DAI Jiejie
Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College; Yunnan Key Laboratory of Major Infectious Diseases Vaccine Development, Kunming 650118, China.
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Affiliation:(1. Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College; Yunnan Key Laboratory of Major Infectious Diseases Vaccine Development, Kunming 650118, China. 2. School of Forensic Medicine, Kunming Medical University, Kunming 650500)
Clc Number:R-33
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Abstract:

Objective Isolation, culture, and identification of dopaminergic neurons from newborn tree shrew to establish a cellular model and study abnormal dopaminergic neurons. Methods B27 and Glutamax were added to culture medium for in vitro culture of dopaminergic neurons from tree shrew. The cultured cells were identified by immunofluorescence. Results Cells isolated from newborn tree shrew were identified as dopaminergic neurons. The rate of tyrosine hydroxylase (TH)?positive neurons was (88.49 ± 2.4)%. Conclusions This method can be used to culture dopaminergic neurons from tree shrew in vitro.

Key words:dopaminergic neurons; primary culture; tree shrew

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Received:March 07,2018
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Online: August 08,2018
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