Effective disinfectants can not only strictly control microorganisms in laboratory animal facilities and ensure the authenticity of experimental result, but also improve the working efficiency and reduce the cost of production. In the daily use of disinfectants, owing to their improper use, the disinfection can be inadequate and even detrimental to the occupational health of employees. This study focused on the use of different kinds of disinfectant in experimental animal facilities, providing a reference for the scientific and rational use of disinfectants for employees.