Establishment of a rat model of acute and chronic fallopian tube inflammatory infertility caused by Staphylococcus aureus

doi:10.3969/j. issn. 1671 -7856. 2018. 08. 011

Home > Archive>Volume 28, Issue 8, 2018 >56-61. DOI:10.3969/j. issn. 1671 -7856. 2018. 08. 011

Establishment of a rat model of acute and chronic fallopian tube inflammatory infertility caused by Staphylococcus aureus
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                        10.3969/j. issn. 1671 -7856. 2018. 08. 011
                    
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                        HU XijiaoHU Xijiao
1. The second hospital of heilongjiang university of traditional Chinese medicine, Harbin 150001,China; 2. Jiamusi college, heilongjiang university of traditional Chinese medicine,Jiamusi 154000; 3. Heilongjiang university of traditional Chinese medicine, Harbin 150040
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LIN ChunshengLIN Chunsheng
1. The second hospital of heilongjiang university of traditional Chinese medicine, Harbin 150001,China; 2. Jiamusi college, heilongjiang university of traditional Chinese medicine,Jiamusi 154000; 3. Heilongjiang university of traditional Chinese medicine, Harbin 150040
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LI ShuoxiLI Shuoxi
1. The second hospital of heilongjiang university of traditional Chinese medicine, Harbin 150001,China; 2. Jiamusi college, heilongjiang university of traditional Chinese medicine,Jiamusi 154000; 3. Heilongjiang university of traditional Chinese medicine, Harbin 150040
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LI YangLI Yang
1. The second hospital of heilongjiang university of traditional Chinese medicine, Harbin 150001,China; 2. Jiamusi college, heilongjiang university of traditional Chinese medicine,Jiamusi 154000; 3. Heilongjiang university of traditional Chinese medicine, Harbin 150040
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LIU LiLIU Li
1. The second hospital of heilongjiang university of traditional Chinese medicine, Harbin 150001,China; 2. Jiamusi college, heilongjiang university of traditional Chinese medicine,Jiamusi 154000; 3. Heilongjiang university of traditional Chinese medicine, Harbin 150040
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Affiliation:(1. The second hospital of heilongjiang university of traditional Chinese medicine, Harbin 150001,China; 2. Jiamusi college, heilongjiang university of traditional Chinese medicine,Jiamusi 154000; 3. Heilongjiang university of traditional Chinese medicine, Harbin 150040)
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Abstract:

Objective To establish a model of acute and chronic oviduct inflammatory infertility in rats infected with Staphylococcus aureus. Methods A 4 × 10⁹ / mL S. aureus suspension was prepared. A total of 108 rats were randomly divided into a normal group, a model group, and a control group, with 36 rats in each. The model group was injected in the fallopian tube with the S. aureus suspension, and the control group was injected with saline in the fallopian tube. The morphology of the fallopian tube was observed on the 7th, 14th, 21st, and 28th days after surgery, and S. aureus culture and pathological examination were also performed. Results After the injection of S. aureus into the fallopian tube, acute oviduct inflammation emerged on postoperative days 7 and 21 to form chronic tubal inflammatory models, and on day 28 to form an oviduct inflammatory model of infertility. Conclusions The injection of 4 ×10⁹ / mL S. aureus into rat oviduct successfully established an acute salpingitis and chronic tubal phlogistic sex infertility rat model.

Key words:fallopian tube inflammatory model; infertility model; Staphylococcus aureus; rat model

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Received:November 29,2017
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Online: September 17,2018
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