Objective To compare and analyze the blood physiological and biochemical indexes of normal Bama minipigs and the F5 generation of a minipig family with hyperglycemia. Methods The fasting blood glucose, postprandial 2 h blood glucose, insulin, glucagon, glycosylated hemoglobin, insulin resistance index, blood physiological index, and blood biochemical index were measured in normal Bama minipigs and the F5 generation of a hyperglycemic family; the intravenous glucose tolerance test was also performed. Results The fasting blood glucose, postprandial 2 h blood glucose, glycated hemoglobin, and insulin resistance index in the hyperglycemic group were significantly higher than those in the normal group ( P < 0. 05). The intravenous glucose tolerance test showed that the fasting blood glucose, 30 min blood glucose level, and 120 min blood glucose level in the hyperglycemic group were significantly higher than those in the normal group ( P < 0. 05). In the blood physiological and biochemical tests, the cholesterol and low?density lipoprotein cholesterol levels in the hyperglycemic group were significantly higher than those in the normal group ( P < 0. 05), but the differences of the other indexes were not pronounced. Conclusions The F5 generation of Bama minipigs showed hyperglycemia and abnormal lipid metabolism.