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Home > Archive>Volume 29, Issue 1, 2019 >84-89. DOI:10.3969/j. issn. 1671 -7856. 2019. 01. 015
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Reproducibility of microsatellite detection for the genetic quality control of laboratory animals
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[cstr]
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Affiliation:

(1. National Institutes for Food and Drug Control, Beijing 102629, China. 2. Zhejiang Academy of Medical Sciences, Hangzhou 310013. 3. Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663)

Clc Number:

R-33

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    Abstract:

    Objective To verify the reproducibility of microsatellite detection for the genetic quality control of laboratory animals in three different laboratories. Methods DNA of six laboratory mice was amplified using 12 microsatellites in three laboratories. PCR products were analyzed by electrophoresis and sequencing. Results Clear electrophoresis bands and 72 alleles were obtained in the Beijing laboratory of National Institutes for Food and Drug Control (NIFDC), the Zhejiang laboratory, and the Guangdong laboratory. Sequencing results showed that the alleles identified were the same in the three different laboratories. Conclusions Microsatellites can be used for the genetic quality control of laboratory animals, with a good reproducibility between different laboratories.

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History
  • Received:March 29,2018
  • Online: February 11,2019
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