LI Zhicao
1. National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique;Center for Tissue Engineering and Stem Cell Research; Department of Pediatrics, Clinical Medical College;Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China.2. Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004.HUANG Bo
National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique;Center for Tissue Engineering and Stem Cell Research; Department of Pediatrics, Clinical Medical College;Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China.XIANG Wenbi
1. National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique;Center for Tissue Engineering and Stem Cell Research; Department of Pediatrics, Clinical Medical College;Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China.2. Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004.SUN Congjie
1. National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique;Center for Tissue Engineering and Stem Cell Research; Department of Pediatrics, Clinical Medical College;Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China.2. Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004.WU Xijun
1. National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique;Center for Tissue Engineering and Stem Cell Research; Department of Pediatrics, Clinical Medical College;Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China.2. Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004.ZHOU Yanhua
1. National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique;Center for Tissue Engineering and Stem Cell Research; Department of Pediatrics, Clinical Medical College;Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China.2. Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004.HE Zhixu
1. Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004.2. Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi 563003SHU Liping
1. National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique;Center for Tissue Engineering and Stem Cell Research; Department of Pediatrics, Clinical Medical College;Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China.2. Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004.(1. National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique;Center for Tissue Engineering and Stem Cell Research; Department of Pediatrics, Clinical Medical College;Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China.2. Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004.3. Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi 563003)
R-33
Objective To evaluate the transplantation effect of erythroid progenitor cells in cloche-/ - mutantzebrafish with congenital primary hematopoietic deficiency. Methods The embryos of zebrafish zTg (gata1:EGFP) werecollected and prepared into a single cell suspension. The gata1+ cells carrying green fluorescence were separated by flowcytometry and transplanted into the hearts of 42 hpf cloche-/ - mutant zebrafish by microinjection. The expression of erythroidprogenitor cells in cloche-/ - mutant zebrafish was examined by stereo fluorescence microscopy. Results gata1+ cells withgreen fluorescence were successfully selected. The proliferation of gata1+ cells with green fluorescence was observed at 2 hafter transplantation, and 16 h of continuous green fluorescence expression was observed. Conclusions Erythroidprogenitor cells have a potential to reconstruct hematopoiesis and provide an experimental basis for the further study of the functional identification of erythroid progenitor cells after transplantation.
