Screening of EV71 pathogenicity-related genes using genetic diversity mice

doi:10.3969/j. issn. 1671 -7856. 2019. 06. 002

Home > Archive>Volume 29, Issue 6, 2019 >8-13. DOI:10.3969/j. issn. 1671 -7856. 2019. 06. 002

Screening of EV71 pathogenicity-related genes using genetic diversity mice
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                        10.3969/j. issn. 1671 -7856. 2019. 06. 002
                    
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Author:
                        CHEN XinCHEN Xin
Comparative Medicine Center,Peking Union Medical College(PUMC);Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences(CAMS); Key Laboratory of Human Diseases Comparative Medicine,National Health Commission; Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious;Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases; Key Laboratory ofHuman Diseases Animal Model, State Administration of Traditional Chinese Medicine;Beijing 100021,China
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Affiliation:(Comparative Medicine Center,Peking Union Medical College(PUMC);Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences(CAMS); Key Laboratory of Human Diseases Comparative Medicine,National Health Commission; Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious;Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases; Key Laboratory ofHuman Diseases Animal Model, State Administration of Traditional Chinese Medicine;Beijing 100021,China)
Clc Number:R-33
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Abstract:

Objective The aim of this study was to screen enterovirus 71(EV71) pathogenicity-related genesusing genetic diversity mice to further understand the pathogenesis of the EV71, and lay the foundation for clinicaldiagnosis, treatment and prognosis. Methods Four-to-six week old genetic diversity mice were challenged with 10⁷copiesof enterovirus 71 by intraperitoneal injection. The blood and hind limb skeletal muscles were collected, and the viral loadwas determined by real-time quantitative PCR. Then, the GeneMine system genetic database was used to analyze theinfection data, and EV71 pathogenicity-related genes were screened. Results Fifty-three genes related to EV71 infectionwere screened using genetic diversity mouse infection data. Conclusions Genetic diversity mice have significantadvantages for simulating population genetic diversity and are a new resource to study complex trait diseases. Mostimportantly, they are an ideal and efficient resource for the screening of pathogenicity-related genes.

Key words:Enterovirus 71; pathogenicity-related host genes; genetic diversity mice; hand, foot and mouth disease

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Received:December 30,2018
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Online: July 16,2019
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