+Advanced Search
Home > Archive>Volume 29, Issue 7, 2019 >29-35. DOI:10.3969/j. issn. 1671 -7856. 2019. 07. 005
PDF HTML XML Export Cite reminder
CCR5 gene mediates migration of myeloid-derived suppressor cells in the mouse liver cancer microenvironment
CSTR:
[cstr]
Author:
Affiliation:

(1. Department of Basic Medicine, Xiamen Medical College, Xiamen 361023, China.2. Key Laboratory of Functional and Clinical Translational medicine, Fujian Province University, Xiamen 361023.3. Institute of Respiratory Diseases Xiamen Medical College, Xiamen 361023)

Clc Number:

R-33

  • Article
    • | |
  • Metrics
    • |
  • Reference
    • |
  • Related
    • |
  • Cited by
    • | |
  • Comments
    Abstract:

    Objective To explore the role of CCR5 gene in the migration of myeloid-derived suppressor cells(MDSCs) into the mouse liver cancer microenvironment. Methods A mouse model of orthotopic liver cancer wasestablished and changes in the MDSCs were detected by flow cytometry. To observe the effect of mouse liver cancer cell H22(T-cell) conditional medium (TCM) on the migration of MDSCs, these cells were sorted and pretreated with 100 nmol/ LCCR5 inhibitor. Finally, DiR-labeled MDSCs were injected into the tail vein of mice with in situ hepatocarcinoma to observethe effect of the CCR5 inhibitor on the migration of MDSCs in vivo. Results The expression of MDSCs in bone marrow,spleen, and liver was significantly higher in the mouse model of orthotopic liver cancer than that in normal mice. The purityof MDSCs was 94. 5%, and MDSCs expressed CCR5. Cell migration result showed that CCR5 inhibitor reduced therecruitment of MDSCs by TCM. The in vivo imaging analysis showed that the ability of MDSCs to migrate to the spleen andliver in vivo was weakened after treatment with CCR5 inhibitor. Conclusions CCR5-mediated MDSCs accumulate in the mouse liver cancer microenvironment. Inhibition of CCR5 in MDSCs may provide a new idea for cancer treatment.

    Reference
    Related
    Cited by
Get Citation

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:January 22,2019
  • Online: August 01,2019
Article QR Code
You are the first Visitors
® 2025 All Rights Reserved
Address：5 Panjiayuan Nanli, Chaoyang District, Beijing 100021, China Postcode： Phone：86-10-67779337 E-mail:bjb@cnilas.org
Supported by:Beijing E-Tiller Technology Development Co., Ltd.