Animal biosafety level-2 (ABSL-2) facilities are used for infected animal experiments. The use of suchfacilities is very high in many scientific research institutions. The efficient management of the facility is a precondition andassurance of animal experiments. In the current article, the operation management experiences of staff in ABSL-2 facilitieswere recorded, such as in the Chinese Center for Disease Control and prevention, and the key points for daily management in such facilities are highlighted to provide a valuable reference.