Cloning a full-length coding sequence of BACE1 in tree shrews and prediction of its molecular characteristics
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(Center of Tree Shrews Germplasm Resource, Institute of Medical Biology, the Chinese Academy of Medical Science Sciences and Peking Union Medical College, Kunming 650118, China)

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R-33

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    Abstract:

    Objective To obtain the full-length coding sequence of β-amyloid precursor protein-cleaving enzyme(BACE1) and analyze its molecular characteristics. Methods Total RNAs from the brains and other organs of tree shrewswere used. The full-length coding sequence of BACE1 was obtained by splicing the sequence obtained via rapid amplificationof cDNA ends (RACE) with the intermediate sequence. Sequence and molecular characteristic analyses were performedusing DNAMAN, MEGA 10. 0. 5, PyMOL and other bioinformatics softwares. Results The BACE1 nucleic acid sequenceof tree shrews was highly similar to that of humans. The overall structures of the two protein molecules were highly similarand had the same extracellular, transmembrane and intracellular domains. Both the tree shrew and the human BACE1proteins had a highly conserved ACDL sorting endocytic motif of DXXLL in the intramembranous domain. However, the treeshrew BACE1 protein had one potential acetylation site compared with that of humans and differed in its folding structure.Conclusions The complete coding sequence of the BACE1 gene is obtained in our study, and the protein structure of treeshrews is similar to that of humans, suggesting that tree shrews may be an ideal model for studying pathological changes of Alzheimer’s disease or potential drug research.

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History
  • Received:June 03,2019
  • Online: September 12,2019
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