Objective To evaluate the antiglioma effect and safety of extracts of the Chinese medicine, Jinlongcapsules (JLC), as well as the crude polysaccharides and proteins prepared from these extracts, to confirm the antigliomaefficacy and safety of the prepared JLC extracts. Methods One hundred 3-4-week old male BALB/ c-nu nude mice weredivided into the following groups: blank control (without tumor, solvent) negative control (solvent), positive control(temozolomide, 32 mg/ kg), low-dose JLC extract (750 mg/ kg), medium-dose JLC extract (1200 mg/ kg), high-doseJLC extract (1500 mg/ kg), crude polysaccharides (100 mg/ kg), and crude protein (100 mg/ kg) groups. An orthotopicsurgical technique was applied to implant a piece of human glioma U87-RFP tumor mass into the brains of nude mice toestablish an orthotopic implantation model of U87-RFP glioma. After building the model, a FluorVivo Model 100fluorescence imaging system was used for noninvasive real-time imaging of the brain tumors in the mice. The effects of JLCextracts on the growth of the orthotopic U87-RFP model were evaluated by comparing the tumor inhibition rates of thesegroups. Results After 25 days of intragastric administration of the drugs, the tumor inhibition rates of the low-dose andmedium-dose groups were both 34. 63%. and neither group’s weight differed significantly from that of the negative controlgroup. For the crude polysaccharide and crude protein groups, the tumor inhibition rates were 22. 26% and 29. 01%, andthe tumor weights were 0. 178±0. 048 g and 0. 144±0. 047 g, respectively, not significantly different from those of thenegative control group. For the positive-control and high-dose groups, the tumor inhibition rates were 99. 57% and61. 90%, and the tumor weights were 0. 001±0. 001 g and 0. 088±0. 045 g, respectively, which differed significantly ( P <0. 01 and P <0. 05, respectively). Conclusions Our findings indicate that crude JLC polysaccharides and proteins have certain inhibiting effect on gliomas. At the doses used in this study, no obvious acute toxic effect is observed.