Establishment of an ELISA assay for the detection of mouse IgG antibody of rabies virus

doi:10.3969/j. issn. 1671 -7856. 2019. 11. 013

Home > Archive>Volume 29, Issue 11, 2019 >85-90. DOI:10.3969/j. issn. 1671 -7856. 2019. 11. 013

Establishment of an ELISA assay for the detection of mouse IgG antibody of rabies virus
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                        10.3969/j. issn. 1671 -7856. 2019. 11. 013
                    
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                        CAI FangzhouCAI Fangzhou
Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS);Comparative Medicine Center, Peking Union Medical College (PUMC); Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases; NHC Key Laboratory of Human Disease Comparative Medicine, Beijing 100021, China
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CHEN QianCHEN Qian
Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS);Comparative Medicine Center, Peking Union Medical College (PUMC); Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases; NHC Key Laboratory of Human Disease Comparative Medicine, Beijing 100021, China
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TONG WeiTONG Wei
Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS);Comparative Medicine Center, Peking Union Medical College (PUMC); Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases; NHC Key Laboratory of Human Disease Comparative Medicine, Beijing 100021, China
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LI DanLI Dan
Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS);Comparative Medicine Center, Peking Union Medical College (PUMC); Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases; NHC Key Laboratory of Human Disease Comparative Medicine, Beijing 100021, China
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WANG WeiWANG Wei
Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS);Comparative Medicine Center, Peking Union Medical College (PUMC); Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases; NHC Key Laboratory of Human Disease Comparative Medicine, Beijing 100021, China
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Affiliation:(Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS);Comparative Medicine Center, Peking Union Medical College (PUMC); Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases; NHC Key Laboratory of Human Disease Comparative Medicine, Beijing 100021, China)
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Abstract:

Objective To establish an ELISA assay for the detection of mouse IgG antibody to rabies virus. Methods Orthogonal experiments were used to determine the optimal dilution of the sample and optimal concentration of the secondary antibody. The stability, specificity and sensitivity test of the assay was determined. The assay result of these samples were compared with those of a commercial kit. Results The optimal dilution of the sample was 1∶100, the optimal concentration of the secondary antibody was 40 ng/ mL, and the cutoff value was 0. 121. There was no crossover with the common mouse viruses, and the minimum detection sensitivity was 129 μg/ mL, and the coefficient variation of the three replicates of the sample was less than 10%. The coincidence rate with the commercial kit was 100%. Conclusions An ELISA assay for the detection of a mouse IgG antibody to rabies virus was successfully established, which can be used for detection and analysis of rabies mouse models.

Key words:rabies virus; IgG Antibody; mouse; ELISA; detection assay

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Online: December 10,2019
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