Objective To explore the mechanism of hepatocarcinogenesis by establishing a C57BL/ 6 chemically induced liver cancer mouse model to simulate the development of liver cancer. Methods A total of 110 C57BL/ 6 mice were randomly divided into a control group ( n = 30) and a model group ( n = 80). The mice in the normal control group were left untreated. The mice in the model group were intraperitoneally injected with DEN followed by the administration of CCl4 orally. The liver morphology was observed, and the levels of alanine aminotransferase ( ALT) and aspartate aminotransferase (AST) in serum were determined by spectrophotometry. HE staining was used to observe pathological changes; the body weight of all groups was also recorded. Results Compared with those in the control group, the body weight of the model group was significantly decreased, as were the levels of ALT and AST. HE staining result showed that the liver of the model group exhibited clear pathological changes. Conclusions Diethylnitrosamine combined with carbon tetrachloride can induce liver cancer in C57BL/ 6 mice, providing a reliable animal model for studying the molecular mechanism of liver cancer development.