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Home > Archive>Volume 30, Issue 2, 2020 >43-53. DOI:10. 3969 / j.issn.1671-7856. 2020. 02. 007
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Comparison of angiogenesis ability and transplantation safety among mesenchymal stem cells derived from umbilical artery, umbilical vein and Wharton’s jelly
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1.Center for Reproductive Medicine, Drum Tower Clinic Medical College of Nanjing Medical University, Nanjing 210008, China. 2. Department of Gynecology, Changzhou First People's Hospital, Changzhou 213000. 3. Experimental Animal Center, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008. 4.Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008. 5.Clinical Center for Stem Cell Research, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008

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R-33

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    Abstract:

    Objective To investigate and compare the angiogenesis ability and transplantation safety of mesenchymal stem cells derived from umbilical artery, umbilical vein and Wharton’ s jelly. Methods We isolated and cultured human umbilical cord artery perivascular stem cells (UCA-PSCs), umbilical cord vein perivascular stem cells (UCV-PSCs) and Wharton’ s jelly mesenchymal stem cells ( WJ-MSCs ). Flow cytometry was used to analyze the expression of cell surface markers at passage 3. The multipotency of cells was analyzed by adipogenic, osteogenic and neural-like differentiation assays. Immunofluorescence was used to detect the perivascular stem cell markers. Angiogenesis ability was analyzed by tube formation assay. Stem cells were transplanted into nonobese diabetic / severe combined immunodeficiency ( NOD-SCID) mice to test the biological safety. Results UCA-PSCs, UCV-PSCs and WJ-MSCs expressed mesenchymal stem cell markers that showed multi-lineage differentiation potential. UCA-PSCs expressed perivascular stem cell markers and had stronger angiogenesis ability in vitro than UCV-PSCs and WJ-MSCs. At 3 months after cell transplantation, hematoxylin and eosin staining showed no obvious morphological damage or abnormality in the organs. Conclusions UCA-PSCs had stronger angiogenesis ability than UCV-PSCs and WJ-MSCs. The transplantation of three kinds of stem cells into NOD-SCID mice result ed in no tumor formation, and appeared to be safe and feasible.

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  • Received:June 28,2019
  • Online: April 01,2020
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