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Home > Archive>Volume 30, Issue 3, 2020 >83-88. DOI:10. 3969 / j.issn.1671-7856. 2020. 03. 014
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Evaluation of the biotyper MALDI-TOF MS system for identification of pathogenic bacteria in laboratory animals
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1.Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 102629, China. 2. Institute for Food and Cosmetics Control, National Institutes for Food and Drug Control, Beijing 100050. 3. College of Veterinary Medicine, China Agricultural University, Beijing 100193

Clc Number:

R-33

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    Abstract:

    Objective To evaluate matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for detection of pathogenic bacteria in laboratory animals. Methods In total, 40 standard strains and 384 laboratory animal isolates were identified using the Bruker Biotyper system. Results The accuracy rates of Bruker Biotyper to detect standard strains and isolates were 90. 0% and 80. 0%, respectively. There were biases in the identification of Salmonella typhimurium, Yersinia pseudotuberculosis, Shigella spp., Klebsiella pneumoniae, Pasteurella pneumotropica biotype Heyl, and Bordetella bronchiseptica. Conclusions The MALDI-TOF MS method is highly efficient and accurate. It can be used for rapid identification of pathogenic bacteria in laboratory animals

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History
  • Received:November 20,2019
  • Online: August 19,2020
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