Preparation of paraffin-embedded sections of zebrafish larva
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Experimental Animal Center of National Research Institute for Family Planning, Beijing 100081, China

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    Abstract:

    Objective To develop a method of paraffin-embedded section preparation of whole zebrafish larva. Methods At 4 days post-fertilization, zebrafish larvae were used to explore the effect of two kinds of fixative solution and dehydration reagent on paraffin-embedded sections. Results Overnight 4% PFA fixation was not suitable for brain or muscle tissues of zebrafish larva. The tissue was severely brittle, but it had little effect on the intestines. Bouin’ s fixative solution was suitable for fixation of zebrafish larva, and subsequent use of tert-butanol or ethanol showed little difference. The dehydration by tert-butanol was slightly better than that of ethanol. For Bouin’ s solution-fixed zebrafish larva, brain tissue dehydrated for 1 minute and muscle tissue dehydrated for 4 minutes can obtain a standard paraffin section which has no folds, no cracks and clearly dyed. Conclusions Under optimized conditions, the prepared sections met the standard for paraffin-embedded sections: a complete, uniform thickness, no folds, no knife marks or cracks, a clearly dyed nucleus, moderately red and blue, transparent, and clean. This method can be used as a reference for researchers who need to prepare paraffin-embedded sections of zebrafish larva.

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History
  • Received:June 18,2020
  • Online: September 02,2020
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