Individual ventilated cage changing standard operating procedure and viral pathogen control in laboratory mice

doi:10. 3969 / j.issn.1671-7856. 2020. 10. 015

Home > Archive>Volume 30, Issue 10, 2020 >107-109. DOI:10. 3969 / j.issn.1671-7856. 2020. 10. 015

Individual ventilated cage changing standard operating procedure and viral pathogen control in laboratory mice
DOI:
                        10. 3969 / j.issn.1671-7856. 2020. 10. 015
                    
CSTR:
                        [cstr]
                    
Author:
                        TONG WeiTONG Wei
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences; Comparative Medical Center, Peking Union Medical College, Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
GUO ZhiGUO Zhi
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences; Comparative Medical Center, Peking Union Medical College, Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
LIU ZiyangLIU Ziyang
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences; Comparative Medical Center, Peking Union Medical College, Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
PAN SidanPAN Sidan
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences; Comparative Medical Center, Peking Union Medical College, Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
GAO HongGAO Hong
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences; Comparative Medical Center, Peking Union Medical College, Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
XIANG ZhiguangXIANG Zhiguang
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences; Comparative Medical Center, Peking Union Medical College, Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site

                    
Affiliation:Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences; Comparative Medical Center, Peking Union Medical College, Beijing 100021, China
Clc Number:R-33
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

Objective To evaluate the effect of the individual ventilated cage (IVC) changing standard operating procedure ( SOP) for viral pathogen transmission in laboratory mice. Methods Six-week-old male BALB/ c mice were infected with mouse hepatitis virus (MHV, A59 strain). The infection status of these infected mice and sentinel mice in close cages on the same shelf, in cages on a different shelf but in the same IVC system, and outside the IVC system but in the same facility were monitored. Serological conversion of MHV antibodies was detected using immunofluorescent antibody reaction and enzyme-linked immunosorbent assay. MHV in mouse feces was detected using reverse transcription polymerase chain reaction. Results MHV nuclear acid was detected 6 d after infection in the artificial infected mice, and serological conversion was detected 3 weeks postinfection. At 5 weeks postinfection, there was no positive detection in sentinel mice. Conclusions The IVC-changing SOP is useful for pathogen transmission management in laboratory mice.

Key words:individual ventilated cages; cage-changing standard operating procedure; mouse hepatitis virus; mouse

Get Citation

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:July 03,2019
Revised:
Adopted:
Online: November 25,2020
Published:

Home

About this journal

Editorial Board

Info for Author

Contact Us

中文版

Get Citation

Share

Article Metrics

History

Article QR Code