Objective To investigate the anti-asthmatic, anti-tussive, and expectorant effects of Tanchuanning mixture. Methods An Asthma model in rats was established by sensitization and challenge with ovalbumin (OVA) and then airway resistance (R_L ) was observed. Serum levels of IgE, IL-4, IL-17, and TNF-α were analyzed by ELISAs. The numbers of WBCs and EOSs in BALF of rats were counted and HE staining of lung tissue was performed to observe pathological changes. Methacholine chloride and histamine phosphate were used to induce asthma in guinea pigs to observe the anti-asthmatic effect of Tanchuanning mixture. The anti-tussive effect of Tanchuanning mixture was observed in a cough mouse model induced by ammonia and guinea pig model induced by citric acid. A phenol red excretion test in the tracheas of mice was used to observe the expectorant effect of Tanchuanning mixture. Results Tanchuanning mixture reduced airway resistance, decreased IgE, IL-4, IL-17, TNF-α levels in serum and the number of WBCs and EOSs in BALF, and alleviated lung tissue damage in asthmatic rats (P<0. 05 or P<0. 01). Tanchuanning mixture prolonged asthma and cough latencies in guinea pigs and mice, respectively, reduced the cough times in mice, and increased phenol red excretion in mice ( P< 0. 05 or P< 0. 01 ). Conclusions Tanchuanning mixture has obvious anti-asthmatic, anti-tussive, and expectorant effects.