Effects of storage conditions on the activity of CD8<sup>+</sup> T cells in rhesus monkeys

doi:10. 3969 / j.issn.1671-7856. 2021. 04. 006

Home > Archive>Volume 31, Issue 4, 2021 >33-37. DOI:10. 3969 / j.issn.1671-7856. 2021. 04. 006

Effects of storage conditions on the activity of CD8+ T cells in rhesus monkeys
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                        10. 3969 / j.issn.1671-7856. 2021. 04. 006
                    
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                        LUO JiahuiLUO Jiahui
Comparative Medicine Center, Peking Union College (PUMC) & Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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LU QiuhanLU Qiuhan
Comparative Medicine Center, Peking Union College (PUMC) & Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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LI GuocuiLI Guocui
Comparative Medicine Center, Peking Union College (PUMC) & Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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ZHANG JingjingZHANG Jingjing
Comparative Medicine Center, Peking Union College (PUMC) & Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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CONG ZheCONG Zhe
Comparative Medicine Center, Peking Union College (PUMC) & Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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WEI QiangWEI Qiang
Comparative Medicine Center, Peking Union College (PUMC) & Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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Affiliation:Comparative Medicine Center, Peking Union College (PUMC) & Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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Abstract:

Objective To use intracellular cytokine staining to detect the intensity of the CD8⁺ T cell response and explore the effects of different storage conditions on CD8⁺ T cell activity. Methods Rhesus macaque peripheral blood was collected and peripheral blood mononuclear cells were separated. Some fresh cells were used directly for analysis and the remaining cells were frozen at - 80℃ or in liquid nitrogen and used for analysis after 1 week or 1 year. A positive stimulus was applied and flow cytometry was used to analyze the proportion of living cells, secretion of MIP-1β, TNF-α, IL-2, and IFN-γ, and expression of cell degranulation marker CD107a. Results As the storage time was increased, the cell survival rate decreased, and liquid nitrogen freezing had a higher survival rate than freezing at -80°C. Secretion of MIP-1β and TNF-α from cells cryopreserved for 1 year was significantly higher than that from normal cells and the cell status had changed. The status of cells frozen for 1 week was more similar to fresh cells. Conclusions To maintain low cell death and a normal CD8⁺ T cell response, fresh cells or short-term frozen cells should be used for experiments to measure the level of the CD8⁺ T cell response.

Key words:CD8⁺ T cells; intracellular cytokine staining; cell viability; rhesus monkeys

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Received:January 08,2021
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Online: May 28,2021
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