Establishment of Marcksl1 gene knockout mice and preliminary analysis of a hematopoietic phenotype
CSTR:
Author:
  • LI Bo

    LI Bo

    1.Beijing Engineering Research Center for Experimental Animal Models of Human Diseases, Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China. 2. Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China (NHC), Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • GAO Lijuan

    GAO Lijuan

    1.Beijing Engineering Research Center for Experimental Animal Models of Human Diseases, Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China. 2. Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China (NHC), Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • YU Lei

    YU Lei

    1.Beijing Engineering Research Center for Experimental Animal Models of Human Diseases, Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China. 2. Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China (NHC), Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • ZHANG Xu

    ZHANG Xu

    1.Beijing Engineering Research Center for Experimental Animal Models of Human Diseases, Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China. 2. Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China (NHC), Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • LI Zhengguang

    LI Zhengguang

    1.Beijing Engineering Research Center for Experimental Animal Models of Human Diseases, Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China. 2. Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China (NHC), Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • LIU Ning

    LIU Ning

    1.Beijing Engineering Research Center for Experimental Animal Models of Human Diseases, Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China. 2. Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China (NHC), Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • SHI Xudong

    SHI Xudong

    1.Beijing Engineering Research Center for Experimental Animal Models of Human Diseases, Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China. 2. Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China (NHC), Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • GAO Ka

    GAO Ka

    1.Beijing Engineering Research Center for Experimental Animal Models of Human Diseases, Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China. 2. Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China (NHC), Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • LI Jing

    LI Jing

    1.Beijing Engineering Research Center for Experimental Animal Models of Human Diseases, Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China. 2. Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China (NHC), Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • GAO Shan

    GAO Shan

    1.Beijing Engineering Research Center for Experimental Animal Models of Human Diseases, Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China. 2. Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China (NHC), Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • QI Xiaolong

    QI Xiaolong

    1.Beijing Engineering Research Center for Experimental Animal Models of Human Diseases, Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China. 2. Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China (NHC), Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • ZHANG Lianfeng

    ZHANG Lianfeng

    1.Beijing Engineering Research Center for Experimental Animal Models of Human Diseases, Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China. 2. Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China (NHC), Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • MA Yuanwu

    MA Yuanwu

    1.Beijing Engineering Research Center for Experimental Animal Models of Human Diseases, Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China. 2. Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China (NHC), Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
Affiliation:

1.Beijing Engineering Research Center for Experimental Animal Models of Human Diseases, Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021, China. 2. Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China (NHC), Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100021

Clc Number:

R-33

  • Article
  • | |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • | | |
  • Comments
    Abstract:

    Objective To generate myristoylated alanine-rich C-kinase substrate-like 1c ( Marcksl1 ) gene knockout mice and investigate the gene function in hematopoiesis. Methods We used CRISPR/ Cas9 technology to produce Marcksl1 gene knockout mice, which was confirmed by PCR and Sanger sequencing. Germline transmission of Marcksl1 gene knockout mice was confirmed by crossing to wild-type mice. We also analyzed the ratio of homozygous, heterozygous, and wild-type mice at different stages of embryonic development. We isolated mice fetal liver to examine the effect of Marcksl1 deletion on the hematopoietic system using routine blood and flow cytometry method . Results PCR and sequencing data showed that Marcksl1 knockout mice were successfully obtained. Our data further suggest that embryonic lethality caused by Marcksl1 gene knockout occurs at a late period of embryonic development. Routine blood and flow cytometry result showed that at E15. 5, deletion of the Marcksl1 gene did not affect the number of white blood cells, red blood cells, or platelets, but the proportion of hematopoietic stem cells in fetal liver increased significantly. Conclusions We have successfully constructed a Marcksl1 knockout mouse. We further found that Marcksl1 deletion increased the number of hematopoietic stem cells. Our work provides a mouse model for further study of the gene function of Marcksl1 in embryonic development and the hematopoietic system.

    Reference
    Related
    Cited by
Get Citation
Share
Article Metrics
  • Abstract:2380
  • PDF: 4140
  • HTML: 0
  • Cited by: 0
History
  • Received:March 01,2020
  • Online: June 25,2021
Article QR Code