Isolation, culture, and characterization of mouse lung-resident mesenchymal stem cells

doi:10. 3969 / j.issn.1671-7856. 2021. 08. 003

Home > Archive>Volume 31, Issue 8, 2021 >16-20. DOI:10. 3969 / j.issn.1671-7856. 2021. 08. 003

Isolation, culture, and characterization of mouse lung-resident mesenchymal stem cells
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                        10. 3969 / j.issn.1671-7856. 2021. 08. 003
                    
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                        SHI GuiyingSHI Guiying
Institute of Laboratory Key Laboratory of Human Disease Sciences, Chinese Academy of Medical Sciences(CAMS); Comparative Medicine Center, Peking Union Medical College(PUMC), Key Laboratory of Human Disease Comparative Medicine, National Health Commission of the People’s Republic of China; Key Laboratory of Human Disease Key Laboratory of Human Disease Models, State Administration of Traditional Chineses Medicine, Beijing 100021, China
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HUANG YiyingHUANG Yiying
Institute of Laboratory Key Laboratory of Human Disease Sciences, Chinese Academy of Medical Sciences(CAMS); Comparative Medicine Center, Peking Union Medical College(PUMC), Key Laboratory of Human Disease Comparative Medicine, National Health Commission of the People’s Republic of China; Key Laboratory of Human Disease Key Laboratory of Human Disease Models, State Administration of Traditional Chineses Medicine, Beijing 100021, China
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LEI XuepeiLEI Xuepei
Institute of Laboratory Key Laboratory of Human Disease Sciences, Chinese Academy of Medical Sciences(CAMS); Comparative Medicine Center, Peking Union Medical College(PUMC), Key Laboratory of Human Disease Comparative Medicine, National Health Commission of the People’s Republic of China; Key Laboratory of Human Disease Key Laboratory of Human Disease Models, State Administration of Traditional Chineses Medicine, Beijing 100021, China
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LU YalanLU Yalan
Institute of Laboratory Key Laboratory of Human Disease Sciences, Chinese Academy of Medical Sciences(CAMS); Comparative Medicine Center, Peking Union Medical College(PUMC), Key Laboratory of Human Disease Comparative Medicine, National Health Commission of the People’s Republic of China; Key Laboratory of Human Disease Key Laboratory of Human Disease Models, State Administration of Traditional Chineses Medicine, Beijing 100021, China
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BAI LinBAI Lin
Institute of Laboratory Key Laboratory of Human Disease Sciences, Chinese Academy of Medical Sciences(CAMS); Comparative Medicine Center, Peking Union Medical College(PUMC), Key Laboratory of Human Disease Comparative Medicine, National Health Commission of the People’s Republic of China; Key Laboratory of Human Disease Key Laboratory of Human Disease Models, State Administration of Traditional Chineses Medicine, Beijing 100021, China
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Affiliation:Institute of Laboratory Key Laboratory of Human Disease Sciences, Chinese Academy of Medical Sciences(CAMS); Comparative Medicine Center, Peking Union Medical College(PUMC), Key Laboratory of Human Disease Comparative Medicine, National Health Commission of the People’s Republic of China; Key Laboratory of Human Disease Key Laboratory of Human Disease Models, State Administration of Traditional Chineses Medicine, Beijing 100021, China
Clc Number:R-33
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Abstract:

Objective To establish a stable method for the isolation, culture, and characterization of mouse lung- resident mesenchymal stem cells, which will then be used to study lung repair and regeneration mechanisms. Methods Mouse lungs were obtained via germ-free procedures and were mechanically minced and / or digested with collagenase. Cell morphology was observed by phase-contrast microscopy, cell surface markers were detected by flow cytometry, and cells were induced to differentiate into adipocytes or osteoblasts. Results We successfully isolated and cultured mouse lung- resident mesenchymal stem cells. FACS analysis demonstrated high expressions of Sca-1, CD90, CD29, and CD44, and low expressions of CD31 and CD45. The lung-resident stem cells differentiated into adipocytes or osteoblasts. Conclusions We isolated cells that expressed mesenchymal stem cell markers and had multi-directional differentiation ability.

Key words:lung-resident mesenchymal stem cell; FACS analysis; induced differentiation

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Received:November 05,2020
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Online: September 26,2021
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