Objective Human SCAＲB2 was identified as the functional receptors of EV71 in vitro，so establish more higher infection ability transgenic mice by establishment of hunman SCAＲB2 transgenic mice in present study．Methods The transgenic vector was constructed by inserting the human SCAＲB2 gene under the CMV promoter and then were subjected to establish transgenic mice by microinjection． The genotype of transgenic line was identified by PCＲ and the expression level of target protein was detected by Western blot． Viral load in the tissues of transgenic mice was detecte by immunohistochemical staining and quantitative real-time PCＲ． Ｒesults One line of transgenic mice in C57BL/6J background with high levels of SCAＲB2 expression in skeletal muscle and brain was identified． Upon infection with EV71，the virus burden of 4 to 5 times in muscle and brain of transgenic mice were significantly higher than that of wild type mice．Conclusion hSCAＲB2 is a functional receptor of EV71 in vivo，as expression of it could promote the infection of EV71 on transgenic mice．