Reactivation effect of NAM on latent reservoir in CD4<sup>+</sup>T cells from SIV / SHIV-infected monkeys

doi:10. 3969 / j.issn.1671-7856. 2022. 05. 004

Home > Archive>Volume 32, Issue 5, 2022 >23-28,46. DOI:10. 3969 / j.issn.1671-7856. 2022. 05. 004

Reactivation effect of NAM on latent reservoir in CD4+T cells from SIV / SHIV-infected monkeys
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                        10. 3969 / j.issn.1671-7856. 2022. 05. 004
                    
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                        DANG CuiDANG Cui
Peking Union Medical College (PUMC) & Institute of Laboratory Animal Science, Comparative Medicine Center, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State administration of Traditional Chinese medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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LU JiahanLU Jiahan
Peking Union Medical College (PUMC) & Institute of Laboratory Animal Science, Comparative Medicine Center, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State administration of Traditional Chinese medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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WEI QiangWEI Qiang
Peking Union Medical College (PUMC) & Institute of Laboratory Animal Science, Comparative Medicine Center, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State administration of Traditional Chinese medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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XUE JingXUE Jing
Peking Union Medical College (PUMC) & Institute of Laboratory Animal Science, Comparative Medicine Center, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State administration of Traditional Chinese medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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CONG ZheCONG Zhe
Peking Union Medical College (PUMC) & Institute of Laboratory Animal Science, Comparative Medicine Center, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State administration of Traditional Chinese medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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Affiliation:Peking Union Medical College (PUMC) & Institute of Laboratory Animal Science, Comparative Medicine Center, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State administration of Traditional Chinese medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
Clc Number:R-33
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Abstract:

Objective To investigate the reactivation effect of nicotinamide (NAM) on latent virus in SIV/ SHIV- infected monkey CD4⁺T cells. Methods Peripheral blood from five SIV/ SHIV-infected rhesus monkeys was collected with undetectable virus loads for at least half a year. CD4⁺ T cells were sorted and treated with latency-reversing agents accompanied by monitoring of viral loads in supernatants and changes in activated T cell surface markers and coreceptors CXCR4 and CCR5 on CD4⁺T cells. Changes in nuclear antigen SIRT1 were detected by Western blot. Results NAM had a good activation effect on latent virus in SIV/ SHIV-infected monkey CD4⁺T cells and reached a higher level combined with GS-9620. This did not cause extensive activation of T cells and had no influence on CCR5 expression, while CXCR4 expression was slightly upregulated. NAM significantly reduced expression of nuclear antigen SIRT1. Conclusions NAM is a good choice for a latency-reversing agent.

Key words:NAM; GS-9620; latency-reversing agent; SIV; SHIV; latent reservoir

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Received:December 22,2021
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Online: July 05,2022
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