Laboratory animals are important in teaching and scientific research, and qualified laboratory animals are an important prerequisite for scientific research. The establishment of scientific and standardized laboratory animal barrier facilities provides specific pathogen-free laboratory animals and platform support to carry out animal research. This article discusses the specific pathogen-free barrier facility for laboratory animals in the School of Pharmaceutical Sciences of Guangzhou University of Chinese Medicine as an example to introduce the operation mode of the laboratory animal barrier facility from the aspects of construction, management of regulations, personnel training and management, sterilization and cleaning, and waste disposal. We also propose relevant solutions to common problems encountered in the management process. Finally, some opinions on the future direction of laboratory animal barrier facilities will be put forward to provide significant information for the construction and management of laboratory animal barrier facility.