Whole-genome analysis and pathogenicity of Aeromonas dhakensis isolated from shrimps

doi:10. 3969 / j.issn.1671-7856. 2023. 03. 004

Home > Archive>Volume 33, Issue 3, 2023 >24-36. DOI:10. 3969 / j.issn.1671-7856. 2023. 03. 004

Whole-genome analysis and pathogenicity of Aeromonas dhakensis isolated from shrimps
DOI:
                        10. 3969 / j.issn.1671-7856. 2023. 03. 004
                    
CSTR:
                        [cstr]
                    
Author:
                        QU FengtianQU Fengtian
1. Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. 2. College of Life Science, Northeast Agricultural University, Haerbin 150000.
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
ZHOU HaijianZHOU Haijian
1. Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
XUE JiaqiXUE Jiaqi
3. Clinic Medical College, North China University of Science and Technology, Tangshan 063000.
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
DU XiaoliDU Xiaoli
1. Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
HU JinruiHU Jinrui
1. Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
CUI ZhigangCUI Zhigang
1. Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
XIE GuilinXIE Guilin
2. College of Life Science, Northeast Agricultural University, Haerbin 150000.
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
LIU ChengengLIU Chengeng
4. Department of Clinical Laboratory, Capital Medical University Beijing Anding Hospital, Beijing 102206
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
MENG ShuangMENG Shuang
1. Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site

                    
Affiliation:1. Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. 2. College of Life Science, Northeast Agricultural University, Haerbin 150000. 3. Clinic Medical College, North China University of Science and Technology, Tangshan 063000. 4. Department of Clinical Laboratory, Capital Medical University Beijing Anding Hospital, Beijing 102206
Clc Number:R-33
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

Objective To investigate the virulence, drug resistance genes, and whole genome information of Aeromonas dhakensis from shrimp and preliminarily evaluate the pathogenicity of Aeromonas dhakensis. Methods Molecular identification and MLST typing of shrimp isolates were performed. The pathogenicity of this strain was examinedby β-hemolysis, extracellular protease, liquid toxicity test, cytotoxicity, and mouse tests, and virulence gene detection. The characteristics of drug resistance were analyzed by a drug sensitivity test and drug resistance gene detection. Eggnog and Prophage Hunter tools were used to analyze the whole genome sequence. Results The 18FX22 strain was identified as Aeromonas dhakensis. The pathogenicity of this strain was characterized by β-hemolysis and extracellular protease activity. It was pathogenic to Caenorhabditis elegans, mouse fibroblasts, and mice. The strain was resistant to amoxicillin/ clavulanic acid, cefazolin, and polymyxin, which carried 10 virulence genes and 12 drug resistance genes. Whole genome sequencing showed that the whole genome size of 18FX22 was 4 811 079 bp and GC content was 61. 33%. COG annotation indicated that 18FX22 had 3850 genes with 20 classes of functions. Prophage prediction revealed that the 18FX22 genome contained two prophage sequences. Cluster analysis of Aeromonas dhakensis worldwide showed that this strain was more closely related to GCA016729585. Conclusions Aeromonas dhakensis was isolated from commercial shrimp and found to be highly toxic, indicating that the control of seafood food safety should be strengthened. This study provides a reference to prevent and control Aeromonas dhakensis infection.

Key words:Aeromonas dhakensis; pathogenicity; whole-genome sequencing; Protophage; COG function analysis

Get Citation

Copy

Article Metrics

Abstract:1759
PDF: 1711
HTML: 0
Cited by: 0

History

Received:July 07,2022
Revised:
Adopted:
Online: April 13,2023
Published:

Home

About this journal

Editorial Board

Info for Author

Contact Us

中文版

Get Citation

Share

Article Metrics

History

Article QR Code