Effects of Yifei moxibustion and its combinations with Tiao bu-Fei shen formulas on MUC5AC and MUC5B in the airways of stable COPD rats

Home > Archive>Volume 33, Issue 4, 2023 >20-27

Effects of Yifei moxibustion and its combinations with Tiao bu-Fei shen formulas on MUC5AC and MUC5B in the airways of stable COPD rats
DOI:
                        
                    
CSTR:
                        [cstr]
                    
Author:
                        LI JingmeiLI Jingmei
1. Henan University of Chinese Medicine, Zhengzhou 450046, China. 2. Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan & Education Ministry of P. R. , Zhengzhou 450046. 4. the Level Three Laboratory of Respiration Pharmacology of TCM in the First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site

                    
Affiliation:1. Henan University of Chinese Medicine, Zhengzhou 450046, China. 2. Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan & Education Ministry of P. R. , Zhengzhou 450046. 3. Henan Key Laboratory of Chinese Medicine for Respiratory Disease, Zhengzhou 450046. 4. the Level Three Laboratory of Respiration Pharmacology of TCM in the First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000
Clc Number:
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

目的探讨益肺灸及其联合调补肺肾三方对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)稳定期大鼠气道黏蛋白5AC(mucin 5AC,MUC5AC)和黏蛋白5B(mucin 5B,MUC5B)水平的影响,阐明益肺灸作用机制和灸药联合是否具备治疗优势。方法采用香烟烟雾暴露联合肺炎克雷伯杆菌感染法建立COPD 稳定期大鼠模型。第9~20 周,空白组和模型(COPD)组给予生理盐水(每只2 mL/ d)、氨茶碱组给予氨茶碱(27 mg/ (kg·d))、补肺健脾组和益肺灸+补肺健脾组给予补肺健脾方(12. 42 g/ (kg·d))、补肺益肾组和益肺灸+补肺益肾组给予补肺益肾方(11. 61 g/ (kg·d))、益气滋肾组和益肺灸+益气滋肾组给予益气滋肾方(12. 42 g/ (kg·d))灌胃,益肺灸组及灸药联合组每周益肺灸灸2 次,每次10~15 min。检测大鼠肺功能、肺组织病理、以及支气管肺泡灌洗液(BALF)和气道中MUC5AC、MUC5B 蛋白表达。结果 COPD 组大鼠较空白组Penh 水平显著升高(P=0. 007),支气管管壁增厚,管腔狭窄,小气道和BALF 中MUC5AC 蛋白表达均显著升高(P<0. 001,P= 0. 005),MUC5B 有升高趋势(P>0. 05)。各治疗干预均显著降低COPD 大鼠Penh 以及BALF 和气道MUC5AC 水平(P<0. 05),益肺灸、调补肺肾三方和益肺灸+益气滋肾方有降低COPD 大鼠MUC5B 的趋势(P>0. 05)。灸药联合在降低Penh、MUC5AC 或MUC5B 气道表达、分泌方面有不同程度上的优于灸、药单独应用的趋势。结论益肺灸、调补肺肾三方及其联合均可改善COPD 大鼠气道通气功能和黏蛋白高表达、分泌,其中,灸药联合可能是更有效策略。

Key words:chronic obstructive pulmonary disease; mucus hypersecretion; MUC5AC; MUC5B; Yifei moxibustion; Tiao bu-Fei shen formulas

Get Citation

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:October 11,2022
Revised:
Adopted:
Online: August 02,2023
Published:

Home

About this journal

Editorial Board

Info for Author

Contact Us

中文版

Get Citation

Share

Article Metrics

History

Article QR Code