Objective To construct a stable and highly feasible rat with hindlimb venous ulcer model, test its physiological and pathological indicators, and provide a standardized animal model for the study of venous leg ulcers. Methods Forty male SD rats were randomly divided into three groups. Fifteen rats in the model group (in which the left common iliac vein was isolated and ligated, and the partial full-thickness of the skin was cut off); 15 in the control group (the left common iliac vein was isolated, and the partial full-thickness of the skin was cut off); and 10 rats in the blank group (no treatment) were observed for 10 days, during which the model rate, general state, venous color Doppler ultrasound images, and changes to the wound surfaces were observed. After 10 days, HE staining was used to observe morphological changes to wound tissue cells. Expression levels of vascular P-selectin and ICAM-1 were detected by immunohistochemistry, and the levels of plasma IL-6 and TNF-α were detected by ELISA. Results (1) Compared with ultrasonography. (2) Compared with the control group, the model group’s wound area was larger (P<0. 05) and the healing rate was lower (P<0. 05). (3) HE staining showed inflammatory cell infiltration in the wound tissue of both the model group and the control group, and the degree of inflammation was more obvious in the model group than the blank group. (4) Immunohistochemistry showed that, compared with the control and blank groups, the model group had significantly up-regulated expression levels of vascular P-selectin and ICAM-1 (P<0. 05). (5) ELISA result showed that, compared with the control and blank groups, the model group’s levels of plasma IL-6 and TNF-α were significantly upregulated (P<0. 05). Conclusions A rat model of hindlimb venous ulcer can be successfully constructed by ligating the left common iliac vein combined with partial full-thickness skin excision.