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Home > Archive>Volume 33, Issue 5, 2023 >61-68. DOI:10. 3969 / j.issn.1671-7856. 2023. 05. 008
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Baicalein protects mouse nucleus pulposus cells from TBHP-induced senescence
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1. Guangzhou University of Chinese Medicine, Guangzhou 510405, China. 2. First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405. 3. Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405

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R-33

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    Abstract:

    Objective To investigate whether Baicalein (BAI) protects nucleus pulposus cells (NPCs) exposed to tert-Butyl hydroperoxide (TBHP). Methods NPCs were extracted from 8-week-old mice and cultured to passages 2 and 3 for experiments in control, model, and baicalein groups. SA-β-galactosidase staining was used to detect senescence of NPCs. Real-time quantitative PCR was used to measure aggrecan, collagen Ⅱ, matrix metalloproteinase-3 (MMP-3), matrix metalloproteinase-9 (MMP-9), tumor necrosis factor α (TNF-α), interleukin (IL)-1β, p53 and p16 mRNA expression. TNF-α was detected by immunofluorescence. Protein expression of aggrecan, p53, p16, IL-1β and TNF-α was detected by Western blot. Results The concentration of TBHP was 100 μmol/ L, and the treatment time was 4 h. The optimal concentration of baicalein was 1 μg/ mL with a treatment time of 24 h. Compared with the control group, the positive rate of SA-β-galactose staining in the TBHP group was significantly increased (P<0. 05), p53, p16, IL-1β and TNF-α mRNA and protein expression was significantly increased ( P<0. 05), and MMP-3 mRNA expression was significantly increased (P<0. 05). MMP-9 mRNA expression was increased, but the difference was not statistically significant (P>0. 05), aggrecan mRNA and protein expression was significantly decreased (P<0. 05), collagen Ⅱ mRNA expression was decreased, but the difference was not statistically significant (P>0. 05), and IL-6 protein expression was increased, but the difference was not statistically significant (P>0. 05). Compared with the model group, the positive rate of SA-β-galactose staining in the baicalein group was significantly lower (P<0. 05). p53, p16, IL-1β, and TNF-α mRNA and protein expression was significantly lower (P<0. 05), and MMP-3 and MMP-9 mRNA expression was significantly lower (P<0. 05). Aggrecan mRNA and protein expression was significantly increased (P<0. 05), collagen Ⅱ mRNA expression was significantly increased (P<0. 05), and IL-6 protein expression was significantly decreased (P<0. 05). Conclusions Baicalein delays senescence of NPCs induced by TBHP.

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History
  • Received:September 27,2022
  • Online: June 15,2023
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